| 辛锦钰,张亚洁,古丽葛娜·萨吾尔,何欢,任珊,安冬青.天香丹对人脐静脉内皮细胞氧化损伤的保护作用研究[J].中医药学报,,50(): |
| 天香丹对人脐静脉内皮细胞氧化损伤的保护作用研究 |
| Protective effect of Tianxiangdan on human umbilical vein endothelial cells injured by oxidative stress |
| 投稿时间：2021-03-01 录用日期：2021-04-26 |
| DOI： |
| 中文关键词: 氧化应激 tBHP 天香丹 人脐静脉内皮细胞 |
| 英文关键词: Oxidative stress tBHP Tianxiangdan human umbilical vein endothelial cells |
| 基金项目:基于“太阳系学说”探讨天香丹调控Nrf2/ARE信号通路改善冠心病冠脉微循环 障碍的机制研究,国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的：探讨天香丹对tBHP诱导损伤的人脐静脉内皮细胞的保护作用。方法：将HUVECs分为对照组、模型组、阳性对照药维生素C组和天香丹剂量组（30、60、90μg/ml）。MTT法筛选tBHP最佳造模浓度，并评价天香丹对内皮细胞氧化损伤的保护作用；微板法和WST法检测各组细胞乳酸脱氢酶（LDH）、还原型谷胱甘肽（GSH）和超氧化物歧化酶（SOD）表达情况；Hoechst 33258荧光染色法检测各组细胞凋亡情况。结果：经tBHP诱导24h后，模型组细胞存活率、SOD活性和GSH含量较对照组显著降低（P<0.01），LDH释放量和细胞凋亡率明显增加（P<0.01）；经维生素C和不同浓度天香丹预处理后，与模型组相比较，各组细胞存活率、SOD活性和GSH含量显著增加，LDH的释放和细胞凋亡率得到减轻（P<0.01）。结论：天香丹具有保护HUVECs免于氧化损伤的作用，其机制可能与抗氧化通路的激活，抗氧化能力的增强及逆转细胞凋亡有关。 |
| 英文摘要: |
| Objective To investigate protective effect of Tianxiangdan on the human umbilical vein endothelial cells injured by tert-butyl hydroperoxide(tBHP). Methods HUVECs were divided into six groups as follows:the control group，the model group, positive drug vitamin C group and Tianxiangdan dosage groups (30, 60, 90μg/ml). The optimal concentration of tBHP was screened by MTT, and the protective effect of Tianxiangdan on endothelial cell oxidative damage was evaluated. Microplate method and WST were used to detect the expression of lactate dehydrogenase (LDH), reduced glutathione (GSH) and superoxide dismutase (SOD) in each group of cells. And apoptosis was observed by Hoechst 33258 fluorescent staining. Results After induction by tBHP for 24 hours, the cell survival rate, the activity of SOD and the level of GSH in the model group were significantly lower than those in the control group (P<0.01), and the release of LDH, apoptosis rate were significantly increased (P<0.01).Compared with the model group，vitamin C and different concentrations of Tianxiangdan significantly increased the cell survival rate, the activity of SOD and the level of GSH，meanwhile the release of LDH and apoptosis rate were reduced (P<0.01). Conculsion Tianxiangdan has the effect of protecting HUVECs from oxidative stress, and its mechanism may be related to the activation of antioxidant pathways, the enhancement of antioxidant capacity and the reversal of cell apoptosis. |
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