糖尿病大鼠中医证候表征的演变

文章摘要

糖尿病大鼠中医证候表征的演变

Evolution of TCM Syndrome Characterization in Diabetic Rats

投稿时间：2020-02-12 录用日期：2020-03-03

DOI：

中文关键词: 糖尿病大鼠中医证候热证阴虚阳虚

英文关键词: diabetic rats TCM syndromes heat syndrome yin deficiency yang deficiency

基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目）

作者	单位	邮编
赵瑛^*	哈尔滨商业大学	150076

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中文摘要:

目的：观察糖尿病（DM）大鼠中医外观体征和证候相关实验室指标的变化特点，分析糖尿病大鼠证候演变规律，为糖尿病证候模型的建立提供思路。方法：腹腔注射65 mg·kg-1链脲佐菌素建立大鼠糖尿病模型，以72h（0w）空腹血糖≥16.7 mmol·L-1作为成模标准。成模第0w、3w、4w、5w和10w观察大鼠外观体征：体质量，毛色毛态，精神状态，行为状态，食量，饮水量，二便情况，舌、爪、鼻特定区域红色程度C值和自主活动能力；并测定对应时间点实验室指标：大鼠血浆葡萄糖、FT3、FT4、cAMP、cGMP、皮质醇含量，计算cAMP/cGMP比值。结果：与对照组相比，DM大鼠在成模0w食量、饮水量和尿量显著升高，持续至10w（P<0.01），体质量、鼻色红色程度C值、自主活动能力、血浆FT3和FT4浓度显著降低，持续至10w（P<0.01）；成模3w血浆cAMP/cGMP显著升高，持续至10w（P<0.05），且出现大便干结呈黑色、毛色枯黄无光泽、毛态竖立症状；成模4w血浆皮质醇浓度显著升高，持续至10w（P<0.01）；成模5w DM大鼠均出现大便干稀不调呈黄色和精神萎靡的症状；第10w舌色红色程度C值显著低于对照组（P<0.01）。结论：以65 mg/kg的剂量腹腔注射链脲佐菌素所建立的糖尿病大鼠10w内模型稳定；早期以内热偏盛为主，STZ可损伤机体阳气，故兼有轻度阳虚表现，且一直贯穿始终。3-5w出现气阴两虚的表现，10w表现为阴阳两虚。

英文摘要:

OBJECTIVE: To observe the characteristics of changes in the appearance and signs of TCM and laboratory indexes related to syndromes in diabetic mellitus (DM) rats, analyze the evolution of syndromes in diabetic rats, and provide ideas for the establishment of diabetes syndrome models. METHODS: Diabetic rat model was established by intraperitoneal injection of 65 mg·kg-1 streptozotocin, and the fasting blood glucose ≥16.7 mmol·L-1 at 72 h (0 week) was used as the model standard. At 0 week(0 w), 3 w, 4 w, 5 w, and 10 w of the model, observe the appearance signs of the rats: body weight, hair color and hair state (soft, withered and yellow, dull, erected, depilated), mental state (normal, debilitating) , behavioral status (normal, less movement, squinting eyes, arched back), food intake, water consumption, urine and stool condition, red degree C value of the tongue, claw and nose in specific areas and ability to move independently; and the laboratory indexes at the corresponding time points were determined: the plasma glucose, FT3, FT4, cAMP, cGMP, and cortisol content of rats, and calculated cAMP/cGMP.RESULTS: Compared with the control group, DM rats significantly increased their food intake, water intake and urine output during model 0w, and continued to 10w (P<0.01). Body weight, nasal color C, autonomic activity, plasma FT3 and The concentration of FT4 decreased significantly and lasted to 10w (P<0.01); the plasma cAMP/cGMP increased significantly during the 3w of the model and continued to 10w (P<0.05), and the dry stool was black, the hair was yellow and dull, and the hair was standing ; Plasma cortisol concentration increased significantly at 4w after modeling, and lasted to 10w (P<0.01); DM rats at 5w after modeling showed symptoms of dryness and thinness of stool and yellowness and malaise; C degree value of tongue redness at 10w It was significantly lower than the control group (P<0.01).CONCLUSION: The model of diabetic rats established by intraperitoneal injection of streptozotocin at a dose of 65 mg/kg is stable within 10 weeks. In the early stage, the internal heat is predominant, and STZ can damage the body's yangqi, so it has mild yang deficiency. and has been throughout. Qi-yin deficiency appears in 3-5 weeks, and yin-yang deficiency in 10 weeks.

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