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| 类叶牡丹皂苷G在大鼠体内的组织分布研究 |
| Study on tissue distribution of Cauloside G from Caulopyhllum robustum in rats |
| 投稿时间：2020-11-24 录用日期：2020-12-01 |
| DOI： |
| 中文关键词: 类叶牡丹 Cauloside G 组织分布 超高效液相色谱-质谱 |
| 英文关键词: Caulopyhllum robustum Cauloside G Tissue distribution UPLC-MS/MS |
| 基金项目:黑龙江省自然科学基金面上项目(H2017063)；黑龙江省普通本科高等学校青年创新人才(UNPYSCT-2016209)；黑龙江省博士后(LBH-TZ20,LBH-Z15209)；黑龙江省博士后启动基金 |
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| 中文摘要: |
| 目的：建立了一种UPLC-MS/MS法,测定类叶牡丹皂苷G(Cauloside G)在大鼠体内各主要脏器(心、肝、脾、肾、脑)中的分布特点及组织药物浓度随时间变化的动态规律。方法：大鼠灌胃给予Cauloside G150 mg/kg,给药后0.5、1、2、4和6 h 5个时间点,收集心、肝、脾、肾、脑组织样品,处理后通过三重四级杆线性离子阱串联质谱仪分析。采用 ACQUITY UPLC HSS T3 (2.1 mm × 150 mm, 1.8 μm)色谱柱,流动相 0.1%甲酸水溶液-0.1%甲酸乙腈溶液,梯度洗脱,流速 0.3mL.min −1,电喷雾离子源,负离子模式,多重反应监测(MRM)。结果：大鼠灌胃给予Cauloside G后,Cauloside G在大鼠的心、肝、脾、肾和脑中均有分布。心脏组织中,Cauloside G是在给药0.5 h后浓度达到最高值,浓度为53.7±7.60 ng/mL；肝、脾、肾、脑组织中,Cauloside G是给药1 h后达到浓度峰值,浓度分别为90.1±6.19 ng/mL,103.2±17.67 ng/mL,101.8±14.32 ng/mL,68.2±14.76 ng/mL。结论：Cauloside G在大鼠体内的各个组织均有分布,其中肝、脾、肾组织中药物浓度较高,该研究为类叶牡丹中三萜皂苷类成分的进一步开发和利用提供参考。 |
| 英文摘要: |
| Objective: To detect the distribution of Cauloside G in the main organs of rats, including the heart, liver, spleen, kidney and brain, and the dynamic changes of drug concentration with time by UPLC-MS/MS. Methods: Rats were intragastrically administrated with 150 mg/kg Cauloside G. At 0.5, 1, 2, 4 and 6 h, rat heart, liver, spleen, kidney, and brain tissues were collected, and subjected to UPLC-MS. The chromatography separation was performed on a ACQUITY UPLC HSS T3 column (2.1 mm × 150 mm, 1.8 μm) with 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile solution for gradient elution (0.3mL.min −1). Heated electrospray ionization source was applied in a negative ion mode for multiple reaction monitoring (MRM). Results: After intragastrical administration of Cauloside G, it was detected to be distributed in rat heart, liver, spleen, kidney, and brain. The concentration of Cauloside G in rat heart peaked at 0.5 h (53.7±7.60 ng/mL). In addition, its concentration in rat liver (90.1±6.19 ng/mL), spleen (103.2±17.67 ng/mL), kidney (101.8±14.32 ng/mL) and brain (68.2±14.76 ng/mL) peaked at 1 h. Conclusion: Cauloside G is distributed in the main organs of rats. Its concentration remains high in rat liver, spleen, and kidney. Our results provide references for developing and using triterpenoid saponins from Caliphyllid robustum in clinical application. |
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