|
| 育阴灵加减方对D-半乳糖所致POI模型大鼠Hippo信号通路的影响 |
| Effect of Yuyinling Modified Formula on Hippo signaling pathway in POI model rats induced by D-galactose |
| 投稿时间：2024-01-04 录用日期：2024-04-15 |
| DOI： |
| 中文关键词: 早发性卵巢功能不全 育阴灵加减方 卵巢结构 Hippo信号通路 |
| 英文关键词: POI Yuyinling Modified Formula Ovary structure Hippo signaling pathway |
| 基金项目:1.黑龙江省人力资源和社会保障厅, 博士后特别资助, LBH-TZ2217, 育阴灵调控内质网应激诱导细胞焦亡在早发性卵巢功能不全治疗中的作用及机制研究； 2.黑龙江省政府, 联合引导, LH2022H079, 育阴灵调控线粒体自噬依赖性铁死亡治疗POI的机制研究； 3.黑龙江省人力资源和社会保障厅, 博士后面上项目, LBH-Z21079, 基于线粒体自噬-NF-κB-NLRP3炎性小体轴探讨育阴灵有效组分对早发性卵巢功能不全的作用及分子机制研究; 4.2023黑龙江省中医药科研项目，ZHY2023-084育阴灵调控内质网应激改善早发性卵巢功能不全的作用机制。 |
|
| 摘要点击次数: 26 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：研究育阴灵加减方对早发性卵巢功能不全（POI）模型大鼠的影响及其可能作用机制。方法：35%D-半乳糖造模成功的POI模型雌性大鼠40只随机分为模型组、中药高（0.562g/kg）、中（0.281g/kg）、低（0.141g/kg）剂量组和西药组，西药组予戊酸雌二醇溶液0.120mg/kg，第4天加灌醋酸甲轻孕酮片溶液0.960mg/kg，随后两药停用1天，为1个疗程，灌胃4个疗程。未造模的8只雌性大鼠作为空白组。观察大鼠体重、卵巢及子宫系数、卵巢组织的病理变化、血清抗缪勒管激素（AMH）、抑制素B（INHB）、血管内皮生长因子（VEGF）水平，蛋白免疫印迹法检法及荧光定量PCR法测定卵巢组织中Hippo信号通路相关效应因子表达情况。结果：与模型组相比中药高、中剂量组可以升高大鼠体重、卵巢系数、子宫系数（P<0.05）；改善卵巢组织结构；升高大鼠血清AMH、INHB、VEGF水平（P<0.05）；Hippo信号通路相关效应因子Mst1、Lats2、YAP1、p-YAP、Smad7的表达量明显降低（P<0.05）。结论：育阴灵加减方可能是通过调控Hippo信号通路的表达起到改善POI模型大鼠卵巢功能的作用。 |
| 英文摘要: |
| Objective: To study the effect of Yuyinling Modified Formula on POI model rats and its possible mechanism. Methods: The POI rat model was established by 35% D-galactose, 40 female rats were randomly divided into model group,Chinese medicine high-dose(0.562g/kg),medium-dose(0.281g/kg),low-dose(0.141g/kg) groups and western medicine group. The western medicine group was given estradiol valerate solution 0.120mg/kg, and megalprogesterone acetate tablet solution 0.960mg/kg, on the 4th day, the two drugs were stopped for 1 day, which was a course of treatment, gavage was administered for 4 courses. Eight unmodeled female rats were used as a blank group. Body weight, ovarian and uterine coefficient, pathological changes of ovarian tissue and serum AMH, INHB and VEGF levels were observed. Western Blot and RT-PCR were used to determine the expression of Hippo signaling pathway in ovarian tissue. Results: Compared with model group, body weight, ovarian coefficient and uterus coefficient of rats in high and medium dose group of Chinese medicine could be increased (P<0.05); Ovarian tissue structure is improved; Serum AMH, INHB and VEGF levels were significantly increased (P<0.05). The expression levels of Hippo signaling pathway related effect factors Mst1, Lats2, YAP1, p-YAP and Smad7 decreased significantly. Conclusion: Yuyinling Modified Formula may improve the ovarian function of POI model rats by regulating the expression of Hippo signaling pathway. |
|
View Fulltext
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
