| Objective: To investigate the effect of Banxia Xiexin Decoction on ferroptosis related molecules in gastric cancer rats induced by MNNG. Methods: 50 SD rats were randomly divided into normal group, GC model group and Banxia Xiexin decoction group. Mice in the normal group were fed normally, and the other two groups were molded by "MNNG gavage + free drinking" to induce the gastric cancer model. The gastric morphology of each group was preliminarily observed; The ggastric pathological histology were observed by hematoxylin-eosin (HE) staining; The expressions of nuclear factor E2-related factor 2 (NRF2), cystine/glutamate reverse transporter solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase (GPX4) and ferritin heavy chain 1 (FTH1) were detected by immunohistochemical assay. Results: At the end of the 52nd week, there were no gastric masses in the normal group, and 4 rats in the GC model group had anterior stomach mass. Two rats in Banxia Xiexin decoction group had Anterior stomach mass; Histopathological observation showed that 4 rats in the model group developed squamous cell carcinoma, 2 rats developed anterior gastric squamous cell papilloma, 1 rats in Banxia Xiexin Tang group developed squamous cell carcinoma and 1 rats developed anterior gastric squamous cell papilloma. Immunohistochemical results showed that the average optical densities of Nrf2, SLC7A11, GPX4 and FTH1 in the model group were significantly higher than those in the normal group (P < 0.05), and the average optical densities of Nrf2, SLC7A11, GPX4 and FTH1 in the model group were significantly lower than those in the model group after Banxia Xiexin decoction intervention (P < 0.05). Conclusion: Banxia Xiexin Decoction can regulate Nrf2, SLC7A11, GPX4 and FTH1 in gastric cancer rats, and induce ferroptosis in gastric cancer. |
