| 金爽,白雪,刘维丽,高萌萌,吕晨,程玉鹏,李慧玲,匡海学.固载乳酸菌发酵中药槐角研究[J].中医药学报,,50():47-51 |
| 固载乳酸菌发酵中药槐角研究 |
| Study on the fermentation of Fructus Sophorae by immobilized Lactic acid bacteria |
| 投稿时间：2021-04-21 录用日期：2021-09-19 |
| DOI： |
| 中文关键词: 固载 乳酸菌 槐角 总黄酮 |
| 英文关键词: Immobilized Lactic acid bacteria Fructus Sophorae Total flavonoids |
| 基金项目:黑龙江省自然科学基金面上项目（H2018053）；中国博士后基金特别资助项目（2018T110321） ；中国博士后基金面上资助项目（2016M591567）；黑龙江省博士后基金资助项目（LBH-Z15206）；黑龙江中医药大学优秀青年教师支持计划项目 |
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| 中文摘要: |
| 目的：采用响应面法优化固载乳酸菌发酵中药槐角发酵体系的最佳条件，为槐角植物药用资源的进一步开发利用提供了参考。方法：以发酵体系中染料木素含量为指标，通过中心组合试验原理设计(Central Composite Design)单因素实验，并结合响应面法考察乳酸菌发酵后pH值、温度和液料比对发酵体系中总黄酮和染料木素含量的影响。结果：得到的最佳发酵条件为pH 5.0，时间36 h，液料比25:1 (mL/g)，优化后槐角发酵体系中总黄酮的含量达到122±0.56 mg/g，染料木素含量18.93±0.47 mg/g为原药材的2.90倍。结论：采用单因素优化结合响应面分析对固载乳酸菌发酵中药槐角体系条件进行优化，发酵后染料木素和总黄酮的含量大大提高，优化后的新工艺可在实际应用中推广，为中药槐角的开发利用奠定了基础，提供了新途径。 |
| 英文摘要: |
| Objective: Response Surface Methodology (RSM) was used to optimize the fermentation conditions of Fructus Sophorae by immobilized Lactic acid bacteria. Methods: The content of total flavonoids in the fermentation system was taken as an index, and a single factor experiment was conducted by Central Composite Design, the effects of pH value, time and liquid-solid ratio on the content of genistein in the fermentation system were investigated by response surface methodology. Results: The optimum processing conditions were pH 5.0, temperature 36 h, liquid-solid ratio 25:1(mL/g) , the content of total flavonoids was 122±0.56 mg/g, and the optimum content of genistein was 18.93 ± 0.47 mg/g, which was 2.90 times to the untreated sample. Conclusion: The single factor and response surface method were used to optimize the fermentation conditions of Fructus Sophorae. with immobilized Lactic acid bacteria.The contents of genistein and total flavonoids were greatly increased in the optimal fermentation condition. The optimized process can be applied in practice, which lays a foundation and provides a new way for the development and utilization of the Fructus Sophorae. |
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